Can I stop my staff from seeing what a booking actually cost me before my markup?

Yes, in most cases you can. Travel agency software is normally set up around user roles, so an owner or manager account can see different information from a counter staff or sales account. When roles are available on your plan, you can usually hide the net cost, the markup amount and the commission from the people who only need to quote and book, while keeping that information visible to you and to whoever handles your accounts.

In practice this means a member of staff opens a booking and sees the selling price, the traveller details, the supplier reference and the cancellation rules, but the cost breakdown is either removed from the screen or replaced with the customer facing total. The same idea applies to reports, so a sales report for staff can show what was sold without showing what each booking earned.

There are honest limits worth knowing. How finely you can control this depends on your plan and on how many roles it offers, so some setups give you a simple staff and admin split rather than a long list of choices. Supplier documents can also leak figures, because a voucher, confirmation email or invoice generated by the supplier may carry a net rate that your software did not create and cannot edit. Staff who hold their own direct login with a supplier will see that supplier's rates regardless of what your portal shows.

The best next step is to ask for a test login at exactly the level you plan to give your team, then make a real booking with it and check every screen, report and attached document to confirm nothing you want private is showing.