Can I set different markups for my walk in customers and my corporate accounts in the same software?

Yes, in most setups you can. Travel agency software is normally built so that markup is not a single number applied to everything. You can usually create separate customer groups, such as walk in retail customers, corporate accounts and sub agents, and give each group its own markup rule. The software then looks at who is logged in or who the booking is being made for, applies the matching rule, and shows that price. This is how agencies sell the same flight or hotel at a retail price on the public site and at a reduced price to a negotiated corporate account without keeping two systems.

How detailed those rules can get depends on your plan and on how your portal is configured. Simple setups allow one markup per group, either a percentage or a flat amount. More detailed setups let you vary the markup by product type, by supplier, by route or destination, by travel class, or by a particular corporate account on its own. Some suppliers also return their own net and published fares differently, so what you can mark up and what you must pass through is partly decided by the supplier rather than by the software. It is worth checking this product by product rather than assuming one rule covers flights, hotels and transfers equally.

One thing to plan for is who is allowed to change these rules. Markup settings usually sit behind an admin permission so that counter staff cannot adjust pricing while making a booking. If you have branches or several people selling, decide early who holds that access.

Before you go live, set up one test corporate account alongside a normal retail search and compare the two prices on the same flight and the same hotel to confirm the rules are landing the way you expect.